CD Skripsi
Optimasi Penyisihan Amonia Oleh Immobilized Microalgal-Bacterial Beads (Imbb) Menggunakan Response Surface Methodology
ABSTRACT
Wastewater often contains pollutants such as ammonia, which can disrupt
ecological balance. This study aimed to determine the optimum conditions,
develop an empirical model, and characterize Immobilized Microalgal–Bacterial
Beads (IMBB) for ammonia removal using Response Surface Methodology (RSM).
The IMBB were formed by immobilizing microalgae Chlorella sp. and bacteria
Nitrosomonas sp. cells in alginate to limit their mobility during batch degradation
of ammonia. The experiments varied ammonia concentrations (50, 175, and 300
mg/L), bacteria-to-microalgae (B/MA) ratios (1:1, 3:1, and 5:1), and contact
times (24, 72, and 120 hours). Under optimum conditions, 165,198 mg/L ammonia
concentration, a bacteria-to-microalgae ratio of 4,431, and a contact time of
81,121 hours, the system achieved ammonia removal efficiency of up to 152,765
mg/L. Characterization using Scanning Electron Microscopy (SEM) revealed a
surface morphological change from smooth to rough, indicating biofilm
development. Energy-Dispersive X-Ray Spectroscopy (EDS) showed a
predominance of carbon, calcium, nitrogen, and oxygen, confirming microbial
and alginate presence. Fourier Transform Infrared (FTIR) spectroscopy identified
hydroxyl, amine, alkyl, alkyne, nitrile, ether, and vinyl functional groups, which
may play roles in ion exchange and adsorption. Mechanical strength decreased
from 0,559 to 0,125 N, while optical density increased from 1,821 to 2,8312,
indicating enhanced microbial growth and metabolic activity. These findings
demonstrate that IMBB offer a sustainable and effective method for ammonia
removal in wastewater treatment systems.
Keywords: ammonia wastewater, immobilized microalgal-bacterial beads,
Chlorella sp.,
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