CD Skripsi
Optimasi Pengolahan Fenol Dengan Immobilized Microalgae Beads Menggunakan Response Surface Methodology (Rsm)
ABSTRACT
Phenol is a toxic aromatic compound commonly found in industrial wastewater and
has the potential to pollute aquatic environments. Treating phenol poses a
challenge due to its resistance to biological degradation, high solubility in water,
and its carcinogenic and bioaccumulative nature. Therefore, an effective and
environmentally friendly treatment method is required. . One of the most effective
methods is biosorption, which utilizes biological biomass such as microalgae to
actively adsorb contaminants through physical and chemical interactions. This
study aims to optimize phenol removal using Immobilized Microalgae Beads (IMB)
based on Chlorella sp. microalgae and alginate as the matrix. Optimization was
carried out using the Response Surface Methodology (RSM) with a Box-Behnken
design, involving initial phenol concentration (mg/L), adsorbent dosage (g/L), and
contact time (hours) as variables. The results showed that the optimal conditions
were achieved at a phenol concentration of 50 mg/L, adsorbent dosage of 9.629
g/L, and contact time of 4.559 hours, with a removal efficiency of 86.59% and an
optical density (OD) value of 2.948. The empirical model yielded a desirability
value of 0.997, indicating a high predictive ability. Characterization of IMB using
SEM, EDS, FTIR, and mechanical strength testing showed supporting results. SEM
revealed an uneven and porous bead surface, enabling optimal diffusion. EDS
identified C, O, Cl, Na, and P as the main elemental components. FTIR analysis
showed the presence of hydroxyl, carboxylate, and polysaccharide groups.
Mechanical strength tests indicated an increase from 57.80 to 70.03 gForce,
suggesting structural stability of the IMB. Overall, IMB has proven to be effective
and stable and holds potential as a sustainable bioremediation technology for
phenol-containing wastewater treatment through efficient biosorption mechanisms.
Keywords: Microalgae Beads; Phenol Removal; RSM; Biosorption; Wastewater
Treatment
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