CD Skripsi
Penggunaan Responses Surface Methodology Untuk Optimasi Penyisihan Amonia Dengan Immobilized Microalgal Chlorella Sp. – Bacterial Nitrosomonas Sp. Beads
ABSTRACT
Ammonia in wastewater can harm aquatic environments due to its toxicity, with
LC50 values of 0.56–2.36 ppm for fish and 1.10–22.8 ppm for invertebrates within
24–96 hours of exposure. It contributes to eutrophication, microbial death, and
reduced dissolved oxygen levels. Conventional are still limited, especially under
high ammonia concentrations. Therefore, this study applied Immobilized
Microalgal Chlorella sp. – Bacterial Nitrosomonas sp. Beads for ammonia
removal. Immobilization provides better control over variables and protects
microorganisms, enhancing removal efficiency. To achieve optimal conditions,
Response Surface Methodology (RSM) was employed, which allows analysis of
individual and interactive effects of variables with minimal experiments. This study
aimed to determine optimal conditions, develop an empirical model for ammonia
removal and Optical Density (OD), and characterize the physical and chemical
properties of the beads using SEM-EDS, FTIR, and mechanical strength testing.
Independent variables included ammonia concentration (250, 375, 500 mg/L), bead
dosage (20, 30, 40 g/L), and bead size (3, 5, 7 mm), with ammonia removal
efficiency and OD as response variables. RSM optimization showed optimal
conditions at 422.651 mg/L ammonia, 37.878 g/L dosage, and 7 mm bead size, with
predicted removal efficiency of 93.83%, final ammonia concentration of 26.08
mg/L, and OD of 3.12. The beads exhibited dense structure (SEM), contained C, O,
Na, Ca (EDS), and functional groups O–H, COO⁻, and C–O–C (FTIR), with
hardness decreasing from 136.30 to 7.62 gForce. These results indicate the
potential of the beads as an environmentally friendly biotechnology solution.
Keywords: IMBB, RSM, Chlorella sp., Nitrosomonas sp., Fisiokimia
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