CD Skripsi
Isolasi Dan Karakterisasi Bakteri Selulolitik Dari Hutan Mangrove Bengkalis: Potensi Sebagai Agen Biofertilizer
Mangrove forests are dynamic coastal ecosystems rich in organic matter and host diverse communities of enzyme-producing microorganisms. Among them, cellulolytic bacteria play a key role in the decomposition of plant biomass by degrading cellulose. This study aimed to isolate and characterize cellulolytic bacteria from the sediments and rhizospheres of natural mangrove plants in Kelapapati Village, Bengkalis District, Riau, Indonesia. Sediment and rhizosphere samples were collected and cultured on Cellulose Congo Red Agar (CCRA) to screen for cellulolytic activity, which was indicated by clear halo zones surrounding bacterial colonies. Isolates with positive cellulolytic activity were further characterized based on their macroscopic and microscopic morphology. A total of 36 bacterial isolates were obtained, consisting of 21 from rhizosphere samples and 15 from sediment. Of these, 20 isolates exhibited cellulolytic activity, with 13 showing high cellulolytic indices, four moderate, and three low. The highest cellulolytic index was observed in isolate MAT 1.1 (10.8 ± 0.6). Most isolates were Gram-negative and exhibited bacillus or streptobacillus morphologies. The physicochemical conditions of the mangrove habitat—pH 6.0, temperature around 30°C, and salinity of 12.67 dS/m—were conducive to the growth of cellulolytic bacteria. These findings highlight the potential of mangrove-derived cellulolytic bacteria as promising candidates for development into biofertilizer agents.
Key findings: Biofertilizer, Cellulase activity, Cellulolytic bacteria, Mangrove, Rhizosphere
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