CD Skripsi

Aktivitas Dekonjugasi Dan Antimikrob Oleh Limosilactobacillus Fermentum Serta Pediococcus Pentosaceus Dengan Atau Tanpa Penggunaan Enkapsulan Dari Batang Kelapa Sawit

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ABSTRACT
Indonesia is the world's largest palm oil producer, generating substantial oil
palm trunks during replanting. These trunks are processed into cellulose microfiber,
which serves as a probiotic encapsulant. This study examined deconjugation and
antimicrobial activity against pathogenic bacteria of Limosilactobacillus fermentum
InaCC B1295 and Pediococcus pentosaceus strain 2397, with or without
encapsulation from oil palm trunk. The experimental research employed a
completely randomized design (CRD) with four treatments: unencapsulated
L. fermentum, unencapsulated P. pentosaceus, encapsulated L. fermentum, and
encapsulated P. pentosaceus. Each treatment was replicated four times, resulting in
16 experimental units. The study assessed bile salt deconjugation on glycocholic
acid and sodium taurocholate, and evaluated the antimicrobial activity of the cellfree
supernatant against both Gram-positive and Gram-negative bacteria. The
observational data obtained were statistically analyzed using analysis of variance
(ANOVA), followed by Duncan’s Multiple Range Test (DMRT) at the 5%
significance level. The results showed that L. fermentum InaCC B1295 and
P. pentosaceus strain 2397 exhibited deconjugation and antimicrobial activities
influenced by the encapsulation process using a CMF hydrogel derived from oil
palm trunk. Deconjugation activity of glycocholic acid was higher than sodium
taurocholate. Encapsulation using CMF hydrogel enhanced enzyme stability and
effectiveness, with the highest antimicrobial activity observed in L. fermentum and
P. pentosaceus against Staphylococcus aureus (12.33 and 10.68 mm, respectively),
and in encapsulated P. pentosaceus against Listeria monocytogenes (13.90 mm).
Overall, encapsulation derived from oil palm trunk influenced the effectiveness of
probiotic in deconjugation and antimicrobial activities, particularly against Grampositive
bacteria.
Keywords: oil palm trunk, Limosilactobacillus fermentum, Pediococcus
pentosaceus, cellulose microfiber, encapsulation.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2106134697

Penerbit

Pekanbaru : Universitas Riau – F.PERTANIAN – TEKNOLOGI PERTANIAN., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2106134697

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

TEKNOLOGI HASIL PERTANIAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

daus

Versi lain/terkait

Lampiran Berkas

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