CD Skripsi
Seleksi Potensi Bakteri Endofit Paku Laut (Acrostichum Aureum) Sebagai Agen Biofertilizer Pada Tanaman Padi
Rice production in Indonesia remains relatively low, partly due to suboptimal fertilization practices. One alternative that can be used is biofertilizer, which contains live microbes that help supply essential nutrients needed by rice plants, such as nitrogen (N), phosphorus (P), and potassium (K). This study aims to select endophytic bacteria from sea ferns originating from Meranti based on their nitrogen fixation ability and their phosphate and potassium solubilization activity. Nitrogen-fixing bacteria were screened using Nitrogen-Free Bromothymol Blue (NFB) medium with a 3-day incubation. Phosphate-solubilizing bacteria were selected using Pikovskaya medium, and potassium-solubilizing bacteria were screened using Alexandrov medium, both incubated for 7 days. Pathogenicity tests were performed on tobacco leaves. Rice seeds were soaked in bacterial inoculum for 2 days to observe their germination response and growth in Petri dishes and peat soil. The results showed that out of 24 endophytic bacterial isolates from Meranti sea ferns, 6 isolates were capable of nitrogen fixation (A.T 1, A.T 2, A.T 3, A.A 3, A.A 4, B.A 3), 4 isolates could solubilize phosphorus (A.T 2, A.T 4, A.A 4, B.A 3), and 3 isolates could solubilize potassium (A.T 1, A.T 2, B.A 3). Pathogenicity testing showed that isolate A.T 2 caused necrosis. Germination and growth tests revealed that isolate A.T 4 improved seed germination, plant height, and root length, while isolate B.A 3 enhanced the dry weight of rice seedlings. Among all isolates, A.T 4 and B.A 3 showed the best potential to be developed as biofertilizer agents for rice cultivation.
Key findings: endophytic bacteria, biofertilizer, rice plant.
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