CD Skripsi

Analisis Homozigositas Tanaman Padi Transgenik Pembawa Gen Biosintesis Lignin Atmyb61

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Plant homozygosity is a condition when alleles in a plant have the same properties. Analysis of the stability of insertion genes in transgenic crops is urgently needed. Transgenics are plants that have been inserted with foreign genes from different plant species or other living things. In this study, the gene inserted was the MYB61 biocystis gene obtained from the Arabidopsis thaliana plant. This is done to ensure that the MYB61 gene is passed on to the next generation in accordance with Mendelian principles. The seeds used in this study are transgenic rice seeds of the Rojolele variety, which have been confirmed to carry the first derivative AtMYB61 gene (T1) which then produces a second derivative plant (T2). Total DNA extraction from rice plant leaf samples was carried out using the CTAB (cetyl trimethylammonium bromide) method. PCR of the AtMYB61 insertion gene was performed by amplifying the Hygromycin phosphotransferase (HPT) gene. The conclusion of the study is that the three strains are based on a 3:1 ratio where ¾ of this ratio can be produced from plant strains that are dominant homozygous and some are heterozogote, while ¼ part of this ratio produces recessive homozygous strains. So, this study produces strains that are dominant homozygous and heterozygous.
Keywords: gene AtMYB61, homozygosity, Hygromycin phosphotransferase, second derivative rice crops (T2), transgenic crops,

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2003113073

Penerbit

Pekanbaru : Universitas Riau FMIPA Biologi., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2003113073

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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