CD Skripsi
Isolasi Dan Seleksi Bakteri Lipolitik Lokal Riau Dan Aplikasinya Dalam Perombakan Minyak Jelantah Menjadi Biodiesel
ABSTRACT
Biodiesel can be used as an alternative to replace diesel fuel produced through transesterification of vegetable oils with alkaline catalysts. Used cooking oil is widely used as a substrate in producing biodiesel due to the process efficiency. In addition to alkaline catalysts, the use of biological catalysts has begun to be developed in accelerating the reaction of biodiesel production. This study aimed to isolate and select Riau's indigenous lipolytic bacteria from hot springs, SBE solid waste and peat soil which are potentially used as catalysts in the biodiesel production from used cooking oil. A total of 9 lipolytic bacterial isolates from the three sample sources were successfully isolated. AP2_60 isolate, extracellular enzymes produced by SBE1_60 isolate, and KOH 5% were used as a biological and chemical catalyst, respectively. The yield percentages of biodiesel produced sequentially were 82.40%, 84.34% and 71.10%. Based on SNI standards for biodiesel namely kinematic viscosity (VK), flash point (TN), acid number (AA) and density (DE), the characters of three produced biodiesel vary. The use of AP2_60 isolate catalyst in biodiesel production met the characteristics of VK, TN and AA and biodiesel from the extracellular enzymes met the characteristics of TN and AA. On the contrary, the use of 5% KOH as a catalyst produced biodiesel that met the characteristics of VK, TN, AA and DE.
Keywords: Biodiesel, catalyst, characterization of biodiesel, lipolytic bacteria.
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