CD Skripsi

Uji Penghambatan Senyawa Metabolit Sekunder Bacillus Subtilis Sebagai Antifungi Terhadap Phytophthora Palmivora Pada Buah Kakao

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ABSTRACT
Phytophthora palmivora is one of the important diseases that cause cacao pod rot disease. This disease can cause production loss up to 90%. P. palmivora control is often carried out using chemical fungicides which often results in the emergence of new problems and environmental pollution, so an environmentally friendly control is needed. This study reported the inhibitory ability of Bacillus subtilis secondary metabolites as antifungals against P. palmivora which causes cocoa pod rot. The experiment was carried out using the poisoned media method by B. subtilis filtrate mixed in potato dextrose agar media. Filtrate was obtained by centrifugation at 10.000 rpm for 15 minutes until the supernatant separated from the precipitate. Data obtained were analyzed statistically by means of variance and further tested by Duncan's New Multiple Range Test (DNMRT). Parameters for observation were virulence tests, the percentage of antifungal inhibition on the growth of Phytophthora palmivora in-vitro, the growth rate of Phytophthora palmivora in-vitro, the percentage of inhibition of the antifungal Bacillus subtilis on the intensity of attacks by Phytophthora palmivora in-vivo. The results obtained were the Phytophthora palmivora is virulent with an intensity of attack of 41.7%. Secondary metabolites of Bacillus subtilis have inhibitory ability as antifungals against Phytophthora palmivora. Concentrations of secondary metabolites of 30% (K3), 40% (K4), and 50% (K5) showed that the concentrations tended to be the best in suppressing Phytophthora palmivora in-vitro are 84.5%, 87.5% and 100% and in-vivo on cocoa pods respectively 83.3%, 91.7% and 100%.
Keywords : cacao pod rot, poisoned media method, secondary metabolites.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1706113401

Penerbit

Pekanbaru : Universitas Riau – Fakultas Pertanian – Agroteknologi., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1706113401

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Agroteknologi

Info Detail Spesifik

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Pernyataan Tanggungjawab

DAUS

Versi lain/terkait

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