CD Skripsi

Analisis Multilokus Empat Barkode Dna Pada Famili Poaceae

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The Poaceae family or the grass family, is a cosmopolitan group of plants with significant benefits in food, livestock feed, and industrial applications. Its high diversity and crucial role present challenges in classification and taxonomy, particularly due to morphological similarities among species. Specific and accurate species identification can be achieved through a molecular approach using DNA barcoding, which has distinct characteristics that determine the accuracy level in species identification. This study aims to test and analyze four types of DNA barcodes and their combinations to determine the most efficient in distinguishing species within the Poaceae family using a bioinformatics approach. The research stages include sequence retrieval from GenBank, alignment, nucleotide base analysis, and phylogenetic tree reconstruction using the Neighbor Joining method with 1000 bootstrap replications. The results show that matK has the highest variation level (82%), while rbcL is the most conserved sequence (86%). Based on phylogenetic tree reconstruction, the single-locus ITS, the two-locus combination ITS + trnL-trnF IGS, the three-locus combinations ITS + matK + trnL-trnF IGS and ITS + rbcL + trnL- trnF IGS, as well as the four-locus combination, demonstrate effective species differentiation within the Poaceae family.
Key findings: Bioinformatics, DNA barcode, multilocus, phylogenetics, poaceae.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2103124387

Penerbit

Pekanbaru : Universitas Riau FMIPA Biologi., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2103124387

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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