CD Skripsi
Uji Potensi Ekstrak Alpukat (Persea Americana Mill.) Sebagai Antioksidan Dan Inhibitor Enzim Α-Amilase
Avocado fruit (Persea americana Mill.) has high nutrition such as healthy fats, fiber, vitamin E and vitamin C. Avocado fruit is also thought to contain many secondary metabolite compounds that can act as antioxidants and inhibitors of the α-amylase enzyme. The samples in this study were avocado varieties of mentega and miki varieties in fresh and dried conditions. Secondary metabolites from this avocado were extracted using two solvents, namely water and n-hexane. Several tests were carried out: water content, percent w/w extracted, phytochemicals, IC50 antioxidants using the DPPH method, and IC50 α-amylase using the DNS method. The results were that the water content of the miki avocado was 86.10% and the mentega avocado was 87.63%. The percentage yield of avocado fruit ranged from 3.12% to 76.9%. Secondary metabolite compounds contained in avocado fruit are alkaloids, phenolics, flavonoids, saponins, steroids and terpenoids. The antioxidant activity of dry butter avocado water extract was 75.42% and dry miki avocado 79.89% and vitamin C as a positive control was 95.53%. The IC50 value of antioxidants from dry butter extract was 77.478 ppm (strong) and dry miki water extract was 21.977 ppm (very strong) and vitamin C was 16.119 (very strong). The activity as an α-amylase inhibitor from n-hexane dry butter extract was 91.15% and dry miki avocado 91.92% and acarbose as a positive control was 99.13%. The IC50 value of n-hexane dry butter extract was 7.845 ppm (very active) and dry miki 15.799 ppm (very active) and acarbose 2.886 ppm (very active). These data show that local avocado varieties of butter and miki have great potential to be used as antioxidants and α-amylase inhibitors to replace vitamin C and the drug acarbose.
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