CD Skripsi
Isolasi Dan Seleksi Bakteri Toleran Logam Berat Pb (Timbal) Dari Tanah Tercemar Limbah Bengkel
The primary source of heavy metal Pb pollution in the environment, which significantly affects water quality and soil engineering properties, is waste from automotive workshops. Bacteria isolated from Pb contaminated environments have shown tolerance to Pb and the ability to accumulate or reduce Pb heavy metal content. This study aimed to isolate and characterize indigenous Pb tolerant bacteria from workshop contaminated soil and to examine their potential for Pb biosorption. Bacteria isolated from workshop soil were partially characterized to identify Pb-tolerant strains. From the tolerant test, one bacterial isolate with the highest Optical Density (OD) value was selected for the biosorption assay. The selected isolate was tested for Pb biosorption using Atomic Absorption Spectroscopy (AAS), and its biosorption efficiency was measured. The results showed that one bacterial isolate from workshop contaminated soil demonstrated Pb tolerance, with isolate BTB 1.4.1 exhibiting the highest OD value. This isolate showed the ability to biosorb Pb heavy metal with an average total biosorption of 68.9%, indicating its tolerance and potential for Pb bioremediation.
Search words: Bioremediation, Indigenous bacteria, Pb, Tolerant, Workshop
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