CD Skripsi

Isolasi Dan Seleksi Bakteri Metanotrof Dari Hutan Mangrove Bengkalis Riau

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Methanotrophic bacteria possess the unique ability to utilize methane (CH₄) as their sole source of carbon and energy. Globally, these bacteria contribute to approximately 10–20% of methane mitigation. This study aimed to isolate and screen methanotrophic bacteria from the mangrove forest in Bengkalis, Riau, using Nitrate Mineral Salt (NMS) Agar as the selective medium. Screening of potential methanotrophs was conducted based on their ability to produce methane monooxygenase (MMO), a key enzyme involved in methane oxidation. Morphological, Gram staining, and catalase activity assessments were performed to characterize the isolates. Five methanotrophic bacterial isolates were successfully obtained. All isolates were Gram-negative and catalase-positive. Colony morphology varied in color (white and pink), size (small to punctiform), elevation (flat to convex), and texture (mucoid to smooth). All isolates shared a circular shape with smooth margins, a glossy surface, and opaque appearance. Cell shape analysis revealed that isolates MA 1, MR 1, and MD 2 were coccoid, while MA 3 and MR 4 were rod-shaped. Enzymatic screening for MMO activity indicated that none of the isolates produced soluble MMO (sMMO), as evidenced by the absence of a purple color change during testing. Instead, all five isolates expressed only the particulate form of MMO (pMMO).

Key findings: CH4, MMO enzyme, Methanotrophic bacteria, NMS media.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2103112292

Penerbit

Pekanbaru : Universitas Riau FMIPA Biologi., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2103112292

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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