CD Skripsi

Deteksi Keanekaragaman Mikroorganisme Dari Sedimen Mangrove Bengkalis, Riau Melalui Pendekatan Next-Generation Sequencing

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Mangrove ecosystems have an important ecological role in maintaining the balance of the coastal environment and becoming a habitat for various microorganisms that support biogeochemical processes. This study aims to detect and analyze the diversity of microorganisms, especially bacteria, from mangrove sediments in Bengkalis Regency, Riau, using a molecular approach through Next-Generation Sequencing (NGS) technology. The sampling locations include three types of mangrove ecosystems, namely natural mangroves, restoration, and degradation. Analysis was carried out on soil physico-chemical parameters, total microorganism populations, and bacterial community structure based on the 16S rRNA gene sequence. The methods used include sediment sampling, environmental characterization, total bacterial and fungal population counting using the Total Plate Count (TPC) method, and metagenomic analysis using the Illumina MiSeq platform. The sequencing data was then analyzed using QIIME2 bioinformatics software with the SILVA database for taxonomic classification. The results show that the physico-chemical characteristics of the soil in each type of mangrove vary and affect the structure of the microorganism community. Natural and restored mangroves have a higher diversity of bacteria compared to degraded mangroves. The dominant bacterial phylum detected include Proteobacteria, Desulfobacterota, Actinobacteriota, Bacteroidota, Firmicutes, Chloroflexi, Campylobacterota, Acidobacteriota, and Myxococcota. The alpha diversity index shows that environmental disturbances due to degradation have an impact on the decline in the diversity of microorganisms. Thus, the NGS approach has proven to be effective in uncovering the community structure of sedimentary microorganisms and can be used as a scientific basis in efforts to restore and manage mangrove ecosystems in a sustainable manner.

Key findings: Bengkalis, bacteria, diversity, mangroves, NGS.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2103112298

Penerbit

Pekanbaru : Universitas Riau FMIPA Biologi., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2103112298

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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