CD Tesis

Identifikasi Dan Aktivitas Antimikroba Bakteri Asam Laktat (BAL) Dari Usus Ikan Patin (Pangasionodon Hypophthalmus) Yang Diberi Pakan Jamu Fermentasi

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The research was carried out starting from rearing fish with fermented
herbal feed in June-August 2019, then identification of bacteria and antimicrobial
activity of LAB was carried out at the Laboratory of Fish Parasites and Diseases,
Department of Aquaculture, Faculty of Fisheries and Marine Science, University
of Riau. Identification of LAB using the PCR (Polymerase Chain Reaction)
technique was carried out at PT. Genetics Science Indonesia, the research was
carried out until April 2020.
The treatment used in this study was with different doses and stocking
density. The dose used, namely; 100 mL/Kg of feed (D1), 200 mL/Kg of feed (D2),
and 300 mL/Kg of feed (D3). The stocking densities used were: 50 fish/m3 (P1)
and 75 fish/m3 (P2). Fish rearing was carried out for 60 days, then isolated and
purified LAB from the intestines of each test fish using the scratch method (streak
quadrant). The results of LAB isolation were tested for antimicrobial activity
against pathogenic bacteria A. hydrophila, E. tarda, and S. aureus using the
paper disk method on NA media. Isolates that have antimicrobial activity were
identified by the PCR method and sequencing carried out by PT. Genetics Science
Indonesian.
A total of seven isolates that were subjected to biochemical tests showed
Gram positive, catalase negative, oxidase negative, and non-motile
characteristics. The antimicrobial activity test results showed that four isolates
were able to inhibit the growth of the test pathogenic bacteria (A. hydrophila, E.
tarda, and S. aureus), namely: Control, P1D2, P2D1, and P2D2. P2D2 isolate
had inhibition against A. hydrophila (7.8 mm), E. tarda (7.3 mm), and S. aureus
(8.8 mm). The results of BLAST sequencing obtained identified bacteria,
including: Lactobacillus casei with 100% homology, Lactobacillus plantarum
99% homology, and two Lactobacillus acidophilus bacteria have a homology
level of 99%.
Key Words : Fermentation Herbs, Pangasionodon hypophthalmus, Pathogenicity,
Lactic Acid Bacteria, Antimicrobials.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1810246188

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Ilmu Kelautan., 2022

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1810246188

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

ILMU KELAUTAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

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