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Isolasi Gen Housekeeping Beta-Tubulin Pada Tumbuhan Durik-Durik (Syzygium Sp.) Dari Danau Kajuik Kabupaten Pelalawan Provinsi Riau

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Durik-durik (Syzygium sp.) is one of the living plants and has adapted to the floodplain area around Kajuik Lake, Riau Province. This plant has an important role as a source of nutrients, animal habitats and prevent erosion in the floodplain lake. This study aims to isolate the Beta-tubulin housekeeping genes in Durik-durik plants (Syzygium sp.) from Kajuik Lake, Pelalawan District. The total DNA molecule was extracted using the Genomic DNA Mini Kit Plant (Geneaid), then amplified using universal Beta-tubulin primer. The PCR product was migrated on 1% agarose gel in 1X TBE buffer. BioEdit Programs Version 7.0, BLASTn, and MEGA Version 6.0 are used for data analysis. The results indicated that the DNA sequence of the Beta-tubulin gene had a size of 1167 bp and encoded 311 amino acids of deduction. The Durik-durik gene sequence had 92% -95% similarity with the Beta-tubulin gene sequence in some plant species.
Keywords: Beta-Tubulin, Durik-durik, Housekeeping Gene, Lake Kajuik, Riau, Syzygium sp.

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Título de la serie

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Localización física

03 04. 118 (0024)

Editorial

Pekanbaru : Universitas Riau – Fakultas Matematika Dan Ilmu Pengetahuan Alam – Biologi., 2018

Descripción física

xii, 30 hlm.; ill.; 29 cm

Idioma

Indonesia

ISBN/ISSN

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Clasificación

03 04. 118 (0024)

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Tipos de medios

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Edición

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Materia(s)

BIOLOGI

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Mención de responsabilidad

DAUS

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