CD Tesis

Kinetika Pertumbuhan Sel Mikroorganisme pada Start-Up Bioreaktor Hibrid Anaerob Menggunakan Substrat Limbah Cair Pabrik Sagu

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The Meranti Islands Regency is renowned as one of the largest sago flour producers in Indonesia, with a total production of 202,186 tons of sago per year and wastewater production reaching 40,000 liters. It is estimated that daily sago wastewater production amounts to 22,156 kL. Biohydrogen can be produced from lignocellulosic wood waste, crude glycerol from the biodiesel industry, cane molasses, and sugar hydrolysate derived from carbohydrate-rich materials such as cassava starch and sago starch. The microorganism Enterobacter aerogenes is a facultative anaerobic hydrogen-producing bacterium through dark fermentation. The kinetics of microbial growth is a key factor in the success of substrate fermentation. The Monod method is one of the alternatives for predicting the kinetics of microorganism growth.
This study aims to determine the kinetics of microorganism cell growth during the start-up phase of a hybrid anaerobic bioreactor, identify the biological oxygen demand (BOD) loading rate during start-up, and evaluate the biological oxygen demand BOD removal efficiency in the start-up phase. The sago wastewater was characterized for its BOD, potential of hydrogen (pH), and volatile suspended solid (VSS) content before treatment. The first phase involved preparing cow dung extract, which serves as a medium for generating microorganisms for biohydrogen production when mixed with sago wastewater. The second phase focused on biomass seeding and acclimatization using a sago medium, maintained at a pH of 5.5 and a temperature of 37℃. The acclimatization process utilized a daily discharge-and-fill method of 200 mL (total acclimatization volume of 12.5 L). Samples from the acclimatization process were analyzed for pH and VSS values. If the acclimatized VSS values were below 10%, the third phase (start-up phase) was initiated. The start-up phase operated under conditions of pH 5.5 and a temperature of 37℃ with a hydraulic retention time (HRT) of 48 hours. Output samples from the start-up phase were analyzed for pH, VSS, and %BOD values until a consistent %BOD value below 10% was achieved, at which point fermentation was halted. The final stage involved data collection for analyzing the kinetics of microorganism cell growth.
The experimental data processing yielded a specific growth kinetics value (µ) of the mixed-culture microorganism cells during the start-up phase, calculated from the linear equation y=−88.741x+162.12y = -88.741x + 162.12y=−88.741x+162.12, with a result of μ=0.00617. The highest BOD loading rate during the start-up phase occurred at the 1st hydraulic retention time (HRT), reaching 6.54 kg/m³/day, accompanied by the lowest BOD removal efficiency of 18.76%. In contrast, the lowest BOD loading rate was recorded at the 11th HRT, at 0.704 kg/m³/day, achieving the highest BOD removal efficiency of 91.26%.
Keywords: Sago Wastewater, BOD, TSS, Bioreactor, Start-Up, Kinetics, and Monod.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2010242025

Penerbit

Pekanbaru : Universitas Riau Pascasarjana Tesis Pendidikan Kimia., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2010242025

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

TEKNIK KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Jaka

Versi lain/terkait

Lampiran Berkas

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