CD Skripsi

Hubungan Polimorfisme Gen Interleukin-10 (-819c/T) Terhadap Status Hbsag Donor Darah Di Utd Pmi Pekanbaru

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Hepatitis B virus (HBV) infection is the main cause of hepatitis which can
progress to cirrhosis and hepatocellular carcinoma (HCC). One of the cytokines
that play an important role in the pathogenesis of HBV is interleukin-10 (IL-10).
IL-10 gene promoter polymorphism can cause changes in IL-10 gene expression,
one of which is polymorphism at the -819 C/T position. This study aims to
analyze the relationship of the IL-10 -819 C/T gene polymorphism on HBsAg
status in blood donors at UTD PMI Pekanbaru. This research was analytic
observational with case-control. The total samples used were 76 samples, 38
samples of reactive HBsAg and 38 samples of non-reactive HBsAg taken from
UTD PMI Pekanbaru. DNA was isolated from whole-blood using the Wizard®
Genomic DNA Purification Kit. The IL-10 -819 gene polymorphism was analysed
by ARMS-PCR. PCR results were subjected to electrophoresis and then
visualization using the Gel Documentation System. The results showed that in
reactive HBsAg cases, the most common genotype distribution was CT (27.6%),
TT (18.4%), CC (3.9%), and in non-reactive HBsAg controls, CT (22.4%), TT
(17.1%), CC (10.5%). The most distribution of alleles for reactive HBsAg were
allele T (63.2%), C (36.8%), and in non-reactive HBsAg, allele T (56.6%), C
(43.4%). There was no significant relationship between genotype polymorphism
and IL-10 -819 allele with HBsAg status of blood donors at UTD PMI Pekanbaru
(p>0.05).
Keywords: Hepatitis B, IL-10 (-819 C/T), HBsAg, Genotype, Allele

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

08 01.121 (0092)

Penerbit

Pekanbaru : Universitas Riau – Kedokteran – Pendidikan Dokter., 2021

Deskripsi Fisik

xiii, 51 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

08 01.121 (0092)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PENDIDIKAN DOKTER

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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