CD Skripsi

Isolasi Dan Uji Aktivitas Antibakteri Senyawa Metabolit Sekunder Dari Ekstrak Diklorometana Tumbuhan Paku Sarang Burung (Asplenium Nidus L.)

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Asplenium nidus commonly known as bird's nest fern, is a member of the Aspleniaceae family and is classified as an epiphytic plant. It has been traditionally used to treat various ailments, including hair loss, fever, headache, cough, bruises, and edema. This plant is known to contain several secondary metabolites such as alkaloids, steroids/terpenoids, flavonoids, and phenolic compounds. The aim of this study was to isolate secondary metabolite compounds from the dichloromethane extract of A. nidus and to evaluate their antibacterial potential. Extraction was performed through maceration using methanol, followed by liquid-liquid partitioning with n-hexane, dichloromethane, and ethyl acetate. Isolation of the compounds was conducted using flash chromatography, which yielded 387 vials that were subsequently combined into 18 fractions based on thin-layer chromatography (TLC) profiles. Fraction 11, which appeared as brown caramel, was subjected to purity testing using TLC and exhibited a single spot at Rf 0.7. The compound's purity was further confirmed by its melting point (79–80 °C) and by high performance liquid chromatography (HPLC), which revealed a single dominant peak. This pure compound was designated as PSB-ESM-F11. Structural characterization using UV-Vis and FTIR spectroscopy identified PSB-ESM-F11 as a phenolic compound. The dichloromethane extract and the isolated compound were tested for antibacterial activity against Staphylococcus aureus, Bacillus cereus, Salmonella typhimurium, and Vibrio parahaemolyticus using the microdilution method. The antibacterial assay showed that both the extract and the PSB-ESM-F11 compound had higher minimum inhibitory concentration (MIC) values against Gram-negative bacteria (S. typhimurium and V. parahaemolyticus) 1000 ppm for the extract and 250 ppm for the compound compared to Gram- positive bacteria (B. cereus and S. aureus), which were inhibited at 250 ppm and 100 ppm, respectively. The minimum bactericidal concentration (MBC) of the dichloromethane extract was only observed for B. cereus at 500 ppm, whereas the PSB-ESM-F11 compound did not exhibit an MBC within the tested concentration range.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2103110586

Penerbit

Pekanbaru : Universitas Riau FMIPA Kimia., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2103110586

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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