Tunjuk langit (Helminthostachys zeylanica (L.) Hook.) is a fern that belongs to the Ophioglossaceae. Phytochemical screening showed that this plant contained secondary metabolites such as flavonoids, terpenoids, phenolics and saponins. The roots of this plant was extracted by maceration method using methanol. Separation of the secondary metabolitesin the methanol extractwere conducted by v…