Research conducted by Gaol et al., (2023) had shown that Penicillium sp. LBKURCC27 was able to produce an orange pigment in potato dextrose broth (PDB) media with a pH of 5.5. As one step to increase the level of production of extracellular pigments and intracellular pigments by Penicillium sp. LBKURCC27, this research had two aims. The first aim was to determine the optimal amount of inoculum…