CD Skripsi

Optimasi Waktu Produksi Metabolit Sekunder Aktinomisetes Asal Tanah Kebun Sayur Di Kota Pekanbaru: Uji Daya Hambat Pertumbuhan Bakteri Patogen

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ABSTRACT
Actinomycetes is a microorganism that can produce secondary metabolites such
as antibiotics. Secondary metabolites can use to control microbial pathogen such
as Staphylococcus aureus, Salmonella typhimurium, Shigella flexneri,
Pseudomonas aeruginosaandEscherichia coli. M1.T1, M1.T3, M1.T4 dan M1.T5
actinomycetes isolates had been isolated from Vegetable Garden Soil of
Pekanbaru city. The purpose of this research is to determine the optimal
secondary metabolite time used by actinomycetes isolate from Vegetable Garden
Soil to inhibit the grows of microbial pathogen. Optimization of secondary
metabolites production time was done by incubation until 14 days. The test
method used to examine antibacterial activity was disc diffusion method.
Antibacterial activity is characterized by the formation of inhibitory zone around
the disc. The result of this study showed that the highest inhibitory zone produced
by M1.T5 isolated in nine day incubation with value of 18,8±0,1 mm against
Staphylococcus aureus. For Salmonella typhimurium bacteriathe highest
inhibitory zone was found in M1.T1isolates in twelve day incubation with value
20,1±0,1 mm. The highest inhibitory zone for Shigella flexneri is found in
M1.T5isolates in seven day incubation with value 19,8±0,3. The highest inhibitory
zone forPseudomonas aeruginosaand Escherichia coli is found in M1.T1in seven
day and ten day incubation with their respective values 16,8±1,1 mm and
15,9±0,5 mm.
Key words: Actinomycetes, secondary metabolites, time optimization.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1603115506

Penerbit

Pekanbaru : Universitas Riau - FMIPA - Biologi., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1603115506

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

TIAR

Versi lain/terkait

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