Antidesma bunius is a plant that belongs to the Euphorbiaceae family. In Indonesia, this plant is known as buni or bonai, which has properties as a traditional medicine. This study aims to isolate and characterize secondary metabolite compounds from ethyl acetate extract of A. bunius leaves, and to conduct toxicity tests using the brine shrimp lethality test (BSLT) method on the extract. Isolat…