This research was conducted in November 2020 to December 2023. The objectives of the research were (1) identify and characterize Chlorella vulgaris isolated from the waters of the Rokan River Estuary, Bangko District, Rokan Hilir Regency, Riau Province, (2) evaluate the effect of different composition media on growth characteristics, proximate chemical composition, chlorophyll (a, b), total chl…