Used plastic bottles can be used for biofilter media, as microorganisms may attach in the bottle surface. To understand the effectiveness of the number of plastic bottles in the anaerob tank in reducing BOD5 and COD in laundry waste, a research has been done in November-December 2019. There were 3 treatments applied, namely P1 (25 bottles, 11.540 cm2 surface area), P2 (50 bottles, 23.080 c…