The research was aimed to observe the using of papain enzyme to remove the limelayer from the skin of sea cucumber (Holothuria scabra) by applying boiling method. The research was using an experimental method and composing as nonfactorial randomized completely design. The treatment conducted was the using of papain enzymes at different concentrations (0, 0.05, 0.1, and 0.15 %) and boiled at…