CD Skripsi

Penambahan Sukrosa Dan Ekstrak Yeast Ke Media Pertumbuhan Bakteri Asam Laktat Terhadap Produksi Antimikroba Lactobacillus Plantarum Tmw 1.1623

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ABSTRACT
Lactic acid bacteria (LAB) are commonly used to preserve food and extend
the shelf life of food. Media de Man Rogosa and Sharpe (MRS) is the medium
commonly used to grow LAB specifically. This study aimed to investigate the
effect of adding yeast extract and sucrose in MRS medium on the Lactobacillus
plantarum TMW 1.1623 acidity value, total LAB, antimicrobial activity of the
LAB, and antimicrobial activity of the supernatant. This research used an
experimental method using a completely randomized design (CRD) with four
treatments and four replications. The treatments were P1 (MRS broth), P2 (MRS
broth + 0.5% yeast extract), P3 MRS broth + 5% sucrose), and P4 (MRS broth +
0.5% yeast extract + 5% sucrose). The data obtained were statistically analyzed
using Analysis of Variance (ANOVA) and Duncan’s Multiple Range Test (DMRT)
at a 5% level. This research showed that treatment P2 with 0.5% yeast extract
addition yielded the best results. The acidity values of the media after incubation
and total LAB were 3.71 and 11.01 (log CFU/mL), respectively. The antimicrobial
activity of LAB measured from the clear zone formed on the media against
Staphylococcus aureus ATCC 25923, Listeria monocytogenes CFSAN 004330, and
Escherichia coli O157:H7 were 14.10, 29.51, and 23.24 mm, respectively. The
clear zone formed due to the antimicrobial activity of the supernatant against
Staphylococcus aureus ATCC 25923, Listeria monocytogenes CFSAN 004330, and
Escherichia coli O157:H7 were 15.94, 25.61, and 18.53 mm, respectively.
Keywords: antimicrobial activity, Lactobacillus plantarum TMW 1.1623, yeast
extract, sucrose

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1506121916

Penerbit

Pekanbaru : Universitas Riau –Fakultas Pertanian – Teknologi Pertanian., 2022

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1506121916

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

TEKNOLOGI PERTANIAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

Daus

Versi lain/terkait

Lampiran Berkas

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