Asplenium nidus Linn is a fern that belongs to the Aspleniaceae family and lives as an epiphyte. Other species from the Aspleniaceae family, such as A. robustum, A. caudatum, and A. tenerum, are known to contain secondary metabolite compounds, incluiding flavonoids, alkaloids, and terpenoids, which exhibit antibacterial, anticancer, and antioxidant activities. The limited information about the …