CD Skripsi

hubungan tingkat metilasi dna dan ekspresi Mrna gen h19 pada pasien oligozoospermia

---

Oligozoospermia, occurs in 34.14% of male infertility cases, define as sperm concentration counted less than 15 million sperm/mL/ejaculation. One of the factor that affects sperm concentration is epigenetic factors, including DNA methylation. We hypotized that sperm concentration is affected by the DNA methylation of H19 gene, a significant gene in fetal growth and development, especially in the process of spermiogenesis. The aim of this study was to analyze DNA methylation and mRNA expression of the H19 gene from male spermatozoas. Methods: Semen samples were collected from 48 males, including 24 normozoospermia and 24 oligozoospermia. DNA methylation and relative mRNA expression of the H19 gene in the sperm cells were examined and analyzed to see the correlation among them.
Results: A significant difference between DNA methylation of the H19 gene in normozoospermia compare to oligozoospermia. In addition, we found that the relative expression of mRNA H19 in oligozoospermia was higher 8.3 times compared to normozoospermia. Moreover, we found there was a negative correlation between the degree of DNA methylation of the H19 gene and the relative expression of its mRNA in normozoospermia males (p=0,002) with r -0,539, but not in the oligozoospermia males (p=0,082) with r -0,362.
Conclusions: There is a significant difference in the DNA methylation level of the H19 gene in normozoospermia and oligozoospermia. There is a significant increase in H19 mRNA expression in oligozoospermia compared to normozoospermia. There is no significant correlation between the DNA methylation level of the H19 gene and the expression of H19 mRNA in oligozoospermia patients. There is a significant correlation between the DNA methylation level of the H19 gene and the expression of H19 mRNA in normozoospermia patients.
Keywords: Male infertility, oligozoospermia, DNA methylation, H19 gene

---

Ketersediaan

Informasi Detail

Judul Seri

-

No. Panggil

2110247810

Penerbit

Pekanbaru : Universitas Riau – PASCA – Magister Ilmu Biomedis., 2025

Deskripsi Fisik

-

Bahasa

Indonesia

ISBN/ISSN

-

Klasifikasi

2110247810

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

Magister Ilmu Biomedis

Info Detail Spesifik

-

Pernyataan Tanggungjawab

Rahmat

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar