ABSTRACT Periphyton is one of microorganisms as function as primary producen in the aquatic environment. Research aims to determine the chlorophyll-ɑ concentration of periphytonattached in plastic pipe substrate has been carried out in April-May 2018 in the Air Hitam River. There were three sampling stations, namely S1 (in the upstream), S2 (in the middle) and S3 (in the downstream).Samplings…