This research was conducted on July until Agustus, 2019 at the Laboratory of Environmental Quality of Culture, Faculty of Fisheries and Marine, University of Riau. Objective of this study to determine the effect of laundry liquid waste on the acute toxicity test and sub lethal of Oreochromis niloticus and safe doses for the growth of O. niloticus. Nile tilapia used in size 5-7 cm. Determination…