Fusarium oxyforum f. sp cubense (Foc TR4) is agent of Fusarium wilt disease that significantly threaten banana existence. Two banana cultivars, awak and lilin, have been previously reported potential resistant against Fusarium wilt disease. NBS- LRR (Nucleotide Binding Site-Leucine Rich Repeat) gene is the largest group of R genes that play an important role in disease resistance. Purpose of th…