Thermophilic bacteria is microbe that can grow at temperatures of 45-80°C and potential in producing enzymes stable to heat. Thermostable enzymes have a number of advantages in their use in the fields of industry and the world of biotechnology. The samples were taken from hot springs in the Pawan area, Rokan Hulu Regency (Rohul), Riau Province. This study uses duplicate repetition of isolation…