Ruku-ruku (Ocimum tenuiflorum) is one species belongs to Lamiaceae family that has a shrub habitus with an average height of 30-60 cm and single-leafed. Ruku- ruku leaves have medicinal properties. This study aims to characterize the anatomical structure of ruku-ruku leaves and determine the location of secondary metabolite compounds contained therein. The methods used were paraffin, paradermal…