CD Skripsi
Aktivitas Antibakteri Ekstrak Rumput Laut Eucheuma Spinosum Terhadap Bakteri Vibrio Alginolyticus Dan Aeromonas Hydrophila
Eucheuma spinosum is a type of red algae (Rhodophyta) that contains
secondary metabolites that have the potential to be used as bioactive producers as
antibacterial. Along with the increase in bacterial resistance to antibiotics, the use
of E. spinosum seaweed as a new antibacterial compound was carried out.
Bacteria Vibrio alginolyticus and Aeromonas hydrophila are aquatic pathogenic
bacteria that can cause various infectious diseases to contaminated living things.
This research was carried out in March – May 2021. This study used an
experimental method compiled using a factorial Completely Randomized Design
(CRD), the first factor was 4 concentrations of E. spinosum extract (A),
concentrations of 100%, 50%, 25% and 12 .5% and the second factor is the type
of pathogenic bacteria (B), i and A. hydrophila. The method used in the
antibacterial activity test was the agar diffusion method or the Kirby-Bauer test
with 3 repetitions and using 40 experimental units as well as positive control from
chloramphenicol antibiotics and negative control from distilled water. Seaweed
extract E. spinosum can act as an antibacterial as evidenced by the presence of
alkaloids and flavonoids in the extract and the formation of an inhibitory zone on
bacterial growth with the highest inhibition zone in V. alginolyticus at a
concentration of 50% with a diameter of 21.03 mm, while in bacteria A. The
highest A. hydrophila inhibition zone was at a concentration of 50% with a
diameter of 25.33 mm.
Keyword: Eucheuma spinosum, Bacterial Activity, Vibrio alginolyticus,
Aeromonas hydrophila.
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