This study aims to determine the effect of different substrates on the spawning and hatching of Zebrafish Pink Danio (Brachydanio rerio), and to determine the best substrate for hatching Zebra Pink Danio eggs. This research was conducted from February to March 2020 at the Fish Hatchery and Breeding Laboratory, Faculty of Fisheries and Marine, Universitas Riau. The fish used were 24 Pink Dan…