Periphyton is a sessile microscopic organism that attach on submerged substrates. A research aims to determine the type and abundance of periphyton attached in the used tyre pieces placed in the Air Hitam River substrate has been carried out in April-May 2018. There were three sampling stations, namely S1 (in the upstream), S2 (in the middle) and S3 (in the downstream). Samplings were conducted…