Gelidium spinosum is one type of red seaweed as raw material for agar because it has gel-forming properties and low sulfate content. Agar is one of the complex polysaccharide hydrocolloids, consisting of 3-linked β-D- galactopynosyl (G) and 4-linked 3,6 anhydro-α-L-galactopynosyl units. Polysaccharides play a role as functional food and neutraceutical. This study aims to determine the physic…