Abstract Laccase (benzenediol:oxygen oxidoreductase, EC 1.10.3.2) is classified as blue copper oxidase enzyme. Laccase generally found in plant, insect, bacteria and filamentous fungus. One of filamentous fungus that able to degradates lignin is Trichoderma. Lignin is available in biomass such as rice straw, that contains around 18% lignin. The technique of producing laccase enzymes in thi…