Penicillium sp. LBKURCC34 is a fungus isolated from peat soil in primary forest, Giam Siak Kecil Bukit Batu Biosphere Reserve (GSKBB), Riau Province. This fungus has potency to produce secondary metabolites that have antimicrobial activity. The study aimed to determine the time for addition of biotic elicitor (Staphylococcus aureus) based on analysis of the secondary metabolite content and acti…