SUMMARY Penicillium sp. LBKURCC34 is a collection isolate of Biochemistry Laboratory, Department of Chemistry. This isolate is originally from Biosphere Reserve in Giam Siak Kecil Bukit Batu (GSKBB) Province of Riau. The fungus produces potential secondary metabolites that are able to inhibit Escherichia coli. The purpose of this study was to purifiy ethyl acetate extract of the liquid media o…