DNA barcoding is a method used to identify species using short DNA sequences from the genome of an organism. One of the DNA barcodes commonly used in molecular analysis is the trnL-trnL-trnF Intergenic Spacer, because it has a high mutation rate and variability. This study aims to analyze the barcode sequence trnL-trnL-trnF Intergenic Spacer in petai (Parkia speciosa) taken in Siak Hulu Di…