Gliocladium sp. T.N.C73 was isolated because it has the ability to produce chitinase. Recent indications that produce several secondary metabolites that antibiotic are soluble in ethyl acetate and methanol. Antibiotic compounds which were suspected to be peptaibols. In this study aimed to compare the antibacterial activity of two concentrations of spores Gliocladium sp. T.N.C73. Antibacterial p…