Helminthostacshys zeylanica is a plant of the family of Ophioglossaceae known as tunjuk langit. The roots of this plant are used as a 100 days cough remedy, dysentery, nasal disease and lung disease. This plant contains saponins and flavonoids. This study aims to isolate and characterize the flavonoid compounds from the roots of this. Plant Isolation methods used was based on polarity fractio…