ABSTRACT Chalcone is one of the secondary metabolite compound of flavonoid group that has C6-C3-C6 as a molecule formula which is known to have diverse biological activities such as antibacterial, antimalarial, antituberkular, anticancer and antioxidant. Chalcone analogue (E)-1-(4-(4-bromobutoxy)phenyl)-3-(2 metoxyphenyl)prop-2-en-1-one (DO1-Br) was synthesized via nucleophilic substitution …