Sour eggplant belongs to the genus Solanum used in this study, which has villosus and not easy to fall out on the skin of the fruit that distinguishes it from other sour eggplant. DNA barcoding is a technique for identification using short DNA sequences from the genome of an organism. The objective of this research is to analyze the trnL-trnL-trnF intergenic spacer of the sour eggplant (S. fero…