Amylase is an enzyme that hydrolyzes of starch into reducing sugars and can be produced from the fermentation process by several microorganisms, including the thermophilic fungus of Aspergillus sp. LBKURCC304. The purposes of this study were to determine the growth curve of Aspergillus sp. LBKURCC304 and optimum biomass from this isolate and study the optimum activity from amylase crude extract…