CD Skripsi
Analisis Keanekaragaman Aksesi Sagu (Metroxylon Spp.) Pada Beberapa Lokasi Menggunakan Start Codon Targeted (Scot)
ABSTRACT
Sago palm (Metroxylon spp.) is a carbohydrate-producing plant that serves as a
staple food in several regions of Indonesia, such as Maluku and Papua. However,
increasing industrial demand has led to excessive exploitation, causing genetic
erosion. Genetic diversity is crucial for ensuring the sustainability of this crop,
contributing to the development of superior varieties and germplasm conservation.
This study aims to analyze the genetic diversity of sago accessions to support
conservation and breeding programs. The research was conducted in the Meranti
Islands using 54 sago accessions from three locations. Genetic diversity was
assessed using start codon targeted (SCoT) markers with primers P33 and P34.
Morphological traits, including plant height, leaf sheath length, spear leaf length,
and stem diameter, were analyzed using ANOVA and Duncan's multiple range
test (5%). Results indicated that location significantly affected morphological
traits except for stem diameter. Molecular analysis identified genetic variation,
with DNA bands ranging from 625 bp to 2000 bp. The C33 location exhibited a
unique genetic pattern, making it a potential source for superior variety
development. Further research is recommended to explore genotype-environment
interactions for optimal sago germplasm management.
Keywords: Genetic diversity, germplasm, leaf sheath length, molecular markers
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