CD Skripsi
Pengaruh Kombinasi Bioflok Dan Manipulasi Fotoperiod Terhadap Perubahan Struktur Darah Pada Ikan Patin (Pangasianodon Hypophthalmus)
Abstract
Pangasianodon hypopthalmus can be reared under biofloc system and short
photoperiod. To understand the effect of biofloc system and photoperiod manipulation
towards blood structure of P. hypopthalmus a study was conducted in September to
December 2020. This experiment uses a factorial CRD design with 2 levels for B factor
(biofloc and no biofloc), 3 levels for P factor (Natural, 18 hours dark, 24 hours dark), 3
repetition. The fish was reared in 110L tank, 30 fishes/tank, fed with fish pellet (FF-
999, Provit 781-1 and Provit 781-2). Parameters measured were the number of
erythrocytes, leukocytes, the level of hematocrit, leukocrit and leukocyte types. The
blood was collected using a syringe wetted with EDTA 10%. The number of blood
cells was calculated using a haemocytometer while the white blood cells were stained
using Giemsa solution and checked under a binocular microscope. Results showed that
there is no different blood structure in fish of all treatments. Total erythrocytes are
1,681,111-2,647,777 cell/mm3, total leukocytes are 55.031-55.655 cell/mm3, hematocrit
20.33-38.11% and leukocrite 20.33-38.11%. White corpuscle are basophil 00-3.89%,
eosinophils 0-3.67%, lymphocytes 26.67-73.89%, trombosit 1-48.89%, monocytes
13.33-30.22% and neutrophil 0.00-9.22%. Based on data obtained, it can be concluded
that the biofloc system and photoperiod manipulation combination do not effect the fish
blood structure.
Keywords : Erythrocytes, Hematocrite, Leukocytes, Leukocrite
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