ABSTRACT Used plastic bottles can be used for biofilter media, as microorganisms may attach in the bottle surface. To understand the effectiveness of the number of plastic bottles in the anaerob tank in reducing MBAS and phosphate in laundry waste, a research has been done in October-December 2019. There were 3 treatments applied, namely P1 (25 bottles, 11.540 cm2 surface area), P2 (50 bo…